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Stimulation of arachidonic acid release by vasopressin in A7r5 vascular smooth muscle cells mediated by Ca 2+ ‐stimulated phospholipase A 2
Author(s) -
Liu Yingjie,
Taylor Colin W.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.055
Subject(s) - arachidonic acid , vasopressin , vascular smooth muscle , stimulation , phospholipase a2 , intracellular , cytosol , phospholipase c , phospholipase , endocrinology , medicine , vasopressin receptor , receptor , biology , chemistry , biochemistry , antagonist , smooth muscle , enzyme
Arachidonic acid (AA) regulates many aspects of vascular smooth muscle behaviour, but the mechanisms linking receptors to AA release are unclear. In A7r5 vascular smooth muscle cells pre‐labelled with 3 H‐AA, vasopressin caused a concentration‐dependent stimulation of 3 H‐AA release that required phospholipase C and an increase in cytosolic [Ca 2+ ]. Ca 2+ release from intracellular stores and Ca 2+ entry via L‐type channels or the capacitative Ca 2+ entry pathway were each effective to varying degrees. Selective inhibitors of PLA 2 inhibited the 3 H‐AA release evoked by vasopressin, though not the underlying Ca 2+ signals, and established that cPLA 2 mediates the release of AA. We conclude that in A7r5 cells vasopressin stimulates AA release via a Ca 2+ ‐dependent activation of cPLA 2 .