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Characterization of chicken interleukin 2 receptor α chain, a homolog to mammalian CD25
Author(s) -
Teng Qiao-Yang,
Zhou Ji-Yong,
Wu Jia-Jun,
Guo Jun-Qing,
Shen Hui-Gang
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.044
Subject(s) - microbiology and biotechnology , il 2 receptor , biology , monoclonal antibody , cd8 , complementary dna , polyclonal antibodies , flow cytometry , virology , antibody , t cell , immune system , gene , immunology , biochemistry
To identify chicken IL‐2R α chain (chCD25), the cDNA of chCD25 was cloned and mapped onto chicken chromosome 1. The polyclonal and monoclonal antibodies raised from the recombinant chCD25 specifically bound to the cell surface of splenic mononuclear cells (SMC) and inhibited chicken IL‐2‐dependent proliferation of T cells. Flow cytometry analysis revealed that chCD25 molecules could be expressed on the surface of monocytes/macrophages, thrombocytes, CD4 + and CD8 + cells as well as tissue cells. Importantly, the CD4 + CD25 + and CD8 + CD25 + cells were upregulated dramatically in chickens infected with H9N2 avian influenza virus. These results confirm that the cloned cDNA is the nucleotide sequence of chicken IL‐2R, and suggest that chicken CD4 + CD25 + and CD8 + CD25 + cells may play an important role in immune responses induced by H9N2 virus, and the monoclonal antibodies to chCD25 may be useful for investigating biological functions of chicken regulatory T cells.