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Enhancement of sapovirus recombinant capsid protein expression in insect cells
Author(s) -
Hansman Grant S.,
Oka Tomoichiro,
Katayama Kazuhiko,
Takeda Naokazu
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.040
Subject(s) - capsid , recombinant dna , sapovirus , virology , protein expression , insect , biology , microbiology and biotechnology , virus , genetics , gene , botany , rotavirus
Human sapovirus (SaV) is uncultivable, but expression of the recombinant capsid protein (rVP1) in insect cells results in the formation of virus‐like particles (VLPs) that are morphologically similar to the native viruses. However, the SaV rVP1 expression levels are considerably low. We have found that inclusions of short foreign nucleotide sequences inserted directly upstream from the predicted rVP1 AUG start codon lead to increased yield of VLPs. This method allowed us to express a SaV rVP1, which could not have been expressed to measurable or practical levels otherwise.