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Mechanisms of ammonium transport, accumulation, and retention in ooyctes and yeast cells expressing Arabidopsis AtAMT1;1
Author(s) -
Wood Craig C.,
Porée Fabien,
Dreyer Ingo,
Koehler Gabriele J.,
Udvardi Michael K.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.026
Subject(s) - vacuole , arabidopsis , yeast , ammonium , mutant , endogeny , transporter , plant cell , ammonia , biochemistry , chemistry , microbiology and biotechnology , arabidopsis thaliana , biology , gene , organic chemistry , cytoplasm
Ammonium is a primary source of N for plants, so knowing how it is transported, stored, and assimilated in plant cells is important for rational approaches to optimise N‐use in agriculture. Electrophysiological studies of Arabidopsis AtAMT1;1 expressed in oocytes revealed passive, Δ ψ ‐driven transport ofNH 4 +through this protein. Expression of AtAMT1;1 in a novel yeast mutant defective in endogenous ammonium transport and vacuolar acidification supported the above mechanism for AtAMT1;1 and revealed a central role for acid vacuoles in storage and retention of ammonia in cells. These results highlight the mechanistic differences between plant AMT proteins and related transporters in bacteria and animal cells, and suggest novel strategies to enhance nitrogen use efficiency in agriculture.