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A normalised scale for structural genomics target ranking: The OB‐Score
Author(s) -
Overton Ian M.,
Barton Geoffrey J.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.015
Subject(s) - ranking (information retrieval) , structural genomics , rank (graph theory) , genomics , quality score , scale (ratio) , isoelectric point , biology , computational biology , statistics , genetics , mathematics , computer science , combinatorics , artificial intelligence , protein structure , gene , biochemistry , genome , geography , cartography , metric (unit) , operations management , economics , enzyme
Target selection and ranking is fundamental to structural genomics. We present a Z‐score scale, the “OB‐Score”, to rank potential targets by their predicted propensity to produce diffraction‐quality crystals. The OB‐Score is derived from a matrix of predicted isoelectric point and hydrophobicity values for nonredundant PDB entries solved to ⩽3.0 Å against a background of UniRef50. A highly significant difference was found between the OB‐Scores for TargetDB test datasets. A wide range of OB‐Scores was observed across 241 proteomes and within 7868 PfamA families; 73.4% of PfamA families contain ⩾1 member with a high OB‐Score, presenting favourable candidates for structural studies.