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A novel function for a ubiquitous plant enzyme pectin methylesterase: The enhancer of RNA silencing
Author(s) -
Dorokhov Yuri L.,
Frolova Olga Y.,
Skurat Eugene V.,
Ivanov Peter A.,
Gasanova Tatjana V.,
Sheveleva Anna A.,
Ravin Nikolay V.,
Mäkinen Kristiina M.,
Klimyuk Victor I.,
Skryabin Konstantin G.,
Gleba Yuri Y.,
Atabekov Joseph G.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.06.013
Subject(s) - rna silencing , nicotiana benthamiana , gene silencing , small interfering rna , rna interference , rna , trans acting sirna , microbiology and biotechnology , rna induced silencing complex , biology , gene expression , cytoplasm , chemistry , gene , biochemistry
Co‐agroinjection of Nicotiana benthamiana leaves with the pectin methylesterase (proPME) gene and the TMV:GFP vector resulted in a stimulation of virus‐induced RNA silencing (inhibition of GFP production, virus RNA degradation, stimulation of siRNAs production). Conversely, co‐expression of TMV:GFP with either antisense PME construct or with enzymatically inactive proPME restored synthesis of viral RNA. Furthermore, expression of proPME enhanced the GFP transgene‐induced gene silencing accompanied by relocation of the DCL1 protein from nucleus to the cytoplasm and activation of siRNAs and miRNAs production. It was hypothesized that DCL1 relocated to the cytoplasm may use as substrates both miRNA precursor and viral RNA. The capacity for enhancing the RNA silencing is a novel function for the polyfunctional PME.