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Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa
Author(s) -
Remminghorst Uwe,
Rehm Bernd H.A.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.077
Subject(s) - periplasmic space , pseudomonas aeruginosa , biosynthesis , biochemistry , mutant , fusion protein , gene , chemistry , function (biology) , biology , escherichia coli , bacteria , recombinant dna , microbiology and biotechnology , genetics
Here the putative alginate biosynthesis gene alg44 of Pseudomonas aeruginosa was functionally assigned. Non‐polar isogenic alg44 deletion mutants of P. aeruginosa were generated and did neither produce alginate nor released free uronic acids. No evidence for alginate enrichment in the periplasm was obtained. Alginate production was restored by introducing only the gene alg44 . PhoA fusion protein analyses suggested that Alg44 is a soluble protein localized in the periplasm. Hexahistidine‐tagged Alg44 was detected by immunoblotting. The corresponding 42.6 kDa protein was purified and identified by MALDI/TOF‐MS analysis. Alg44 might be directly involved in alginate polymerization presumably by exerting a regulatory function.