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A novel nuclear‐localized protein with special adenylate kinase properties from Caenorhabditis elegans
Author(s) -
Zhai Ruitong,
Meng Geng,
Zhao Yanmei,
Liu Bin,
Zhang Genfa,
Zheng Xiaofeng
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.074
Subject(s) - caenorhabditis elegans , adenylate kinase , rna interference , subcellular localization , gene isoform , biology , protein kinase a , biochemistry , escherichia coli , enzyme , kinase , microbiology and biotechnology , chemistry , rna , gene
The adrenal gland protein AD‐004 like protein (ADLP) from Caenorhabditis elegans was cloned and expressed in Escherichia coli . Enzyme assays showed that ADLP has special adenylate kinase (AK) properties, with ATP and dATP as the preferred phosphate donors. In contrast to all other AK isoforms, AMP and dAMP were the preferred substrates of ADLP; CMP, TMP and shikimate acid were also good substrates. Subcellular localization studies showed a predominant nuclear localization for this protein, which is different from AK1–AK5, but similar to that of human AK6. These results suggest that ADLP is more likely a member of the AK6 family. Furthermore, RNAi experiments targeting ADLP were conducted and showed that RNAi treatment resulted in the suppression of worm growth.