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Silencing of the human microsomal glucose‐6‐phosphate translocase induces glioma cell death: Potential new anticancer target for curcumin
Author(s) -
Belkaid Anissa,
Copland Ian B.,
Massillon Duna,
Annabi Borhane
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.071
Subject(s) - curcumin , gene silencing , propidium iodide , small interfering rna , cancer research , programmed cell death , biology , cancer cell , apoptosis , glioma , microbiology and biotechnology , cancer , biochemistry , transfection , gene , genetics
G6P translocase (G6PT) is thought to play a crucial role in transducing intracellular signaling events in brain tumor‐derived cancer cells. In this report, we investigated the contribution of G6PT to the control of U‐87 brain tumor‐derived glioma cell survival using small interfering RNA (siRNA)‐mediated suppression of G6PT. Three siRNA constructs were generated and found to suppress up to 91% G6PT gene expression. Flow cytometry analysis of propidium iodide/annexin‐V‐stained cells indicated that silencing the G6PT gene induced necrosis and late apoptosis. The anticancer agent curcumin, also inhibited G6PT gene expression by more than 90% and triggered U‐87 glioma cells death. Overexpression of recombinant G6PT rescued the cells from curcumin‐induced cell death. Targeting G6PT expression may provide a new mechanistic rationale for the action of chemopreventive drugs and lead to the development of new anti‐cancer strategies.