Identification of the cytochrome P450 enzymes responsible for the ω‐hydroxylation of phytanic acid | Zendy

Komen J.C. | Zendy; Wanders R.J.A. | Zendy

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Identification of the cytochrome P450 enzymes responsible for the ω‐hydroxylation of phytanic acid

Author(s) -

Komen J.C.,

Wanders R.J.A.

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.05.069

Subject(s) - phytanic acid , hydroxylation , cytochrome p450 , enzyme , biochemistry , chemistry , microsome , recombinant dna , peroxisome , gene

Patients suffering from Refsum disease have a defect in the α‐oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the ω‐oxidation pathway. With the use of specific inhibitors we now show that members of the cytochrome P450 (CYP450) family 4 class are responsible for phytanic acid ω‐hydroxylation. Incubations with microsomes containing human recombinant CYP450s (Supersomes™) revealed that multiple CYP450 enzymes of the family 4 class are able to ω‐hydroxylate phytanic acid with the following order of efficiency: CYP4F3A > CYP4F3B > CYP4F2 > CYP4A11.

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