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Novel potential of tunicamycin as an activator of the aryl hydrocarbon receptor – dioxin responsive element signaling pathway
Author(s) -
Horikawa Kyohei,
Oishi Naoki,
Nakagawa Jin,
Kasai Ayumi,
Hayakawa Kunihiro,
Hiramatsu Nobuhiko,
Takano Yosuke,
Yokouchi Makiko,
Yao Jian,
Kitamura Masanori
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.061
Subject(s) - aryl hydrocarbon receptor , chemistry , activator (genetics) , tunicamycin , signal transduction , receptor , microbiology and biotechnology , biochemistry , apoptosis , biology , transcription factor , unfolded protein response , gene
Tunicamycin is a well‐known inhibitor of protein glycosylation and used as an inducer of endoplasmic reticulum (ER) stress. We found that tunicamycin induced expression of cytochrome P450 1A1 in a dose‐dependent manner. Like dioxin, the transcriptional induction was associated with dose‐dependent activation of the dioxin responsive element (DRE). This effect was independent of inhibition of protein glycosylation or induction of ER stress. Pharmacological and genetic inhibition of the aryl hydrocarbon receptor (AhR) significantly attenuated activation of DRE by tunicamycin. These results elucidated the novel potential of tunicamycin as an activator of the AhR – DRE signaling pathway.