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Intravenous administration of mannosylated cationic liposome/NFκB decoy complexes effectively prevent LPS‐induced cytokine production in a murine liver failure model
Author(s) -
Higuchi Yuriko,
Kawakami Shigeru,
Oka Machiko,
Yabe Yoshiyuki,
Yamashita Fumiyoshi,
Hashida Mitsuru
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.059
Subject(s) - cationic liposome , liposome , decoy , lipopolysaccharide , cytokine , tumor necrosis factor alpha , pharmacology , chemistry , inflammation , medicine , immunology , biochemistry , transfection , receptor , gene
The purpose of this study was to inhibit endotoxin induced cytokines production and liver injury by liver non‐parenchymal cell (NPC) selective delivery of nuclear factor κB (NFκB) decoy using mannosylated cationic liposomes (Man‐liposomes). In this study, we examined the distribution, inhibitory effect on cytokines production and ALT/AST of intravenously injected Man‐liposome/NFκB decoy complex. Man‐liposome/[ 32 P] NFκB decoy complexes mostly accumulated in the liver, preferentially in NPC. In a murine lipopolysaccharide‐induced liver failure model, the production of tumor necrosis factor‐α (TNFα), IFNγ, IL1‐β, ALT and AST were effectively reduced by Man‐liposome complexes. However, cationic or galactosylated cationic liposome complexes could not inhibit TNFα production.