A novel apoptosis cascade mediated by lysosomal lactoferrin and its participation in hepatocyte apoptosis induced by  d ‐galactosamine | Zendy

Katunuma Nobuhiko | Zendy; Le Quang Trong | Zendy; Murata Etsuko | Zendy; Matsui Atsushi | Zendy; Majima Eiji | Zendy; Ishimaru Naozumi | Zendy; Hayashi Yoshio | Zendy; Ohashi Atsushi | Zendy

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A novel apoptosis cascade mediated by lysosomal lactoferrin and its participation in hepatocyte apoptosis induced by d ‐galactosamine

Author(s) -

Katunuma Nobuhiko,

Le Quang Trong,

Murata Etsuko,

Matsui Atsushi,

Majima Eiji,

Ishimaru Naozumi,

Hayashi Yoshio,

Ohashi Atsushi

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.05.057

Subject(s) - lactoferrin , apoptosis , cytoplasm , chemistry , microbiology and biotechnology , hepatocyte , caspase , in vitro , biology , biochemistry , programmed cell death

A new apoptosis cascade mediated by lysosomal lactoferrin was found in apoptotic liver induced by d ‐galactosamine. Caspase‐3 and lactoferrin were increased in the apoptotic liver cytoplasm and serum transaminases were elevated. Recombinant lactoferrin stimulated procaspase‐3 processing at 10 −6 –10 −7 M to an extent similar to that by granzyme B in vitro. Lactoferrin changed procaspase‐3 structure susceptible to the processing. Synthetic peptide Y 679 ‐K 695 in lactoferrin molecule inhibited the processing of procaspase‐3 by lactoferrin. Lactoferrin in lysosomes was decreased and lactoferrin released into cytoplasm was increased quantitatively in d ‐galactosamine induced apoptotic liver, and procaspase‐3 in cytoplasm was processed to caspase‐3.

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