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A novel apoptosis cascade mediated by lysosomal lactoferrin and its participation in hepatocyte apoptosis induced by d ‐galactosamine
Author(s) -
Katunuma Nobuhiko,
Le Quang Trong,
Murata Etsuko,
Matsui Atsushi,
Majima Eiji,
Ishimaru Naozumi,
Hayashi Yoshio,
Ohashi Atsushi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.057
Subject(s) - lactoferrin , apoptosis , cytoplasm , chemistry , microbiology and biotechnology , hepatocyte , caspase , in vitro , biology , biochemistry , programmed cell death
A new apoptosis cascade mediated by lysosomal lactoferrin was found in apoptotic liver induced by d ‐galactosamine. Caspase‐3 and lactoferrin were increased in the apoptotic liver cytoplasm and serum transaminases were elevated. Recombinant lactoferrin stimulated procaspase‐3 processing at 10 −6 –10 −7 M to an extent similar to that by granzyme B in vitro. Lactoferrin changed procaspase‐3 structure susceptible to the processing. Synthetic peptide Y 679 ‐K 695 in lactoferrin molecule inhibited the processing of procaspase‐3 by lactoferrin. Lactoferrin in lysosomes was decreased and lactoferrin released into cytoplasm was increased quantitatively in d ‐galactosamine induced apoptotic liver, and procaspase‐3 in cytoplasm was processed to caspase‐3.