Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from  E. coli | Zendy

Yamamoto Tatsuya | Zendy; Izumi Shunsuke | Zendy; Gekko Kunihiko | Zendy

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Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli

Author(s) -

Yamamoto Tatsuya,

Izumi Shunsuke,

Gekko Kunihiko

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.05.049

Subject(s) - ribosome , hydrogen–deuterium exchange , deuterium , ribosomal protein , chemistry , mass spectrometry , transfer rna , escherichia coli , ribosomal rna , rna , biophysics , biochemistry , chromatography , biology , physics , gene , quantum mechanics

The 70S ribosome from Escherichia coli is a supermacro complex (MW: 2.7 MDa) comprising three RNA molecules and more than 50 proteins. We have for the first time successfully analyzed the flexibility of 70S ribosomal proteins in solution by detecting the hydrogen/deuterium exchange with mass spectrometry. Based on the deuterium incorporation map of the X‐ray structure obtained at the time of each exchange, we demonstrate the structure–flexibility–function relationship of ribosome focusing on the deuterium incorporation of the proteins binding ligands (tRNA, mRNA, and elongation factor) and the relation with structural assembly processes.

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