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Silencing of HIV by hairpin‐loop‐structured DNA oligonucleotide
Author(s) -
Moelling Karin,
Abels Susanne,
Jendis Joerg,
Matskevich Alexey,
Heinrich Jochen
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.033
Subject(s) - oligonucleotide , gene silencing , dna , human immunodeficiency virus (hiv) , chemistry , computational biology , biology , microbiology and biotechnology , genetics , virology , gene
We describe inhibition of HIV replication by a partially double‐stranded 54mer oligodeoxynucleotide, ODN, which consists of an antisense strand targeting the highly conserved polypurine tract, PPT, of HIV, and a second strand, compatible with triple‐helix formation. Upon treatment of HIV‐infected cells with ODN early after infection no viral nucleic acids, syncytia or p24 viral antigen expression was observed. The ODN‐mediated effect was highly sequence‐specific. The ODN against HIV‐IIIB was effective preferentially against its homologous PPT and less against the PPT of HIV‐BaL differing in two of 24 nucleotides and vice versa. It may be interesting mechanistically as an antiviral drug.