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The neural cell adhesion molecule binds to fibroblast growth factor receptor 2
Author(s) -
Christensen Claus,
Lauridsen Jes B.,
Berezin Vladimir,
Bock Elisabeth,
Kiselyov Vladislav V.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.008
Subject(s) - neural cell adhesion molecule , fibroblast growth factor receptor 1 , fibroblast growth factor receptor , receptor , fibroblast growth factor receptor 4 , microbiology and biotechnology , fibroblast growth factor , chemistry , fibroblast growth factor receptor 2 , gene isoform , cell adhesion , biology , biochemistry , cell , gene
The neural cell adhesion molecule (NCAM) can bind to and activate fibroblast growth factor receptor 1 (FGFR1). However, there are four major FGFR isoforms (FGFR1–FGFR4), and it is not known whether NCAM also interacts directly with the other three FGFR isoforms. In this study, we show by surface plasmon resonance analysis that NCAM can bind to FGFR2 with an affinity similar to that for the NCAM–FGFR1 interaction. However, the kinetic parameters for the NCAM–FGFR2 binding are different from those of the NCAM–FGFR1 binding. Both receptors were shown to cycle relatively fast between the NCAM bound and unbound states, although FGFR2 cycling was clearly faster (13 times) than the FGFR1 cycling. Moreover, ATP was more effective in inhibiting the binding of NCAM to FGFR1 than to FGFR2, indicating that the binding sites in NCAM for the two receptors are similar, but not identical.