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Upregulation of thromboxane synthase in human colorectal carcinoma and the cancer cell proliferation by thromboxane A 2
Author(s) -
Sakai Hideki,
Suzuki Tomoyuki,
Takahashi Yuji,
Ukai Masashi,
Tauchi Katsunori,
Fujii Takuto,
Horikawa Naoki,
Minamimura Tetsuji,
Tabuchi Yoshiaki,
Morii Magotoshi,
Tsukada Kazuhiro,
Takeguchi Noriaki
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.007
Subject(s) - thromboxane a synthase , downregulation and upregulation , cyclooxygenase , colorectal cancer , cancer research , cell growth , cancer , thromboxane , prostaglandin h2 , thromboxane a2 , cancer cell , biology , arachidonic acid , chemistry , biochemistry , medicine , enzyme , immunology , platelet , receptor , gene
Tumor growth of colorectal cancers accompanies upregulation of cyclooxygenase‐2, which catalyzes a conversion step from arachidonic acid to prostaglandin H 2 (PGH 2 ). Here, we compared the expression levels of thromboxane synthase (TXS), which catalyzes the conversion of PGH 2 to thromboxane A 2 (TXA 2 ), between human colorectal cancer tissue and its accompanying normal mucosa. It was found that TXS protein was consistently upregulated in the cancer tissues from different patients. TXS was also highly expressed in human colonic cancer cell lines. Depletion of TXS protein by the antisense oligonucleotide inhibited proliferation of the cancer cells. This inhibition was rescued by the direct addition of a stable analogue of TXA 2 . The present results suggest that overexpression of TXS and subsequent excess production of TXA 2 in the cancer cells may be involved in the tumor growth of human colorectum.