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POSH, a scaffold protein for JNK signaling, binds to ALG‐2 and ALIX in Drosophila
Author(s) -
Tsuda Manabu,
Seong Ki-Hyeon,
Aigaki Toshiro
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.005
Subject(s) - microbiology and biotechnology , scaffold protein , imaginal disc , signal transduction , phenotype , apoptosis , ectopic expression , kinase , programmed cell death , gene , biology , chemistry , genetics
Plenty of SH3s (POSH) functions as a scaffold protein for the Jun N‐terminal kinase (JNK) signal transduction pathway, which leads to cell death in mammalian cultured cells and Drosophila . Here, we show that POSH forms a complex with Apoptosis‐linked gene‐2 (ALG‐2) and ALG‐2‐interacting protein (ALIX/AIP1) in a calcium‐dependent manner. Overexpression of ALG‐2 or ALIX in developing imaginal eye discs resulted in roughened or melanized eyes, respectively. These phenotypes were enhanced by co‐overexpression of POSH. We found that overexpression of either gene could induce ectopic JNK activation, suggesting that POSH/ALG‐2/ALIX may function together in the regulation of the JNK pathway.