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Interaction of pro‐apoptotic protein HGTD‐P with heat shock protein 90 is required for induction of mitochondrial apoptotic cascades
Author(s) -
Kim Jee-Youn,
Kim Su-Mi,
Ko Jeong-Hun,
Yim Ji-Hye,
Park Jin-Hae,
Park Jae-Hoon
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.05.001
Subject(s) - apoptosis , microbiology and biotechnology , heat shock protein , chemistry , mitochondrion , biology , biochemistry , gene
HGTD‐P is a hypoxia‐responsive pro‐apoptotic protein that transmits hypoxic signals directly to mitochondria. When overexpressed, HGTD‐P induces cell death via typical mitochondrial apoptotic cascades. However, much is unknown about post‐transcriptional modification and signaling networks of HGTD‐P in association with cell death‐regulating proteins. We performed yeast two‐hybrid screening to identify the molecules involved in HGTD‐P‐mediated cell death pathways. In this study, we show that heat shock protein 90 physically interacts with HGTD‐P and that suppression of Hsp90 activity by low concentrations of geldanamycin reduced HGTD‐P‐induced mitochondrial catastrophe through inhibition of mitochondrial translocation of HGTD‐P.