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Analysis of leptin signalling in hematopoietic cells using an adapted MAPPIT strategy
Author(s) -
Montoye T.,
Piessevaux J.,
Lavens D.,
Wauman J.,
Catteeuw D.,
Vandekerckhove J.,
Lemmens I.,
Tavernier J.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.094
Subject(s) - leptin , socs3 , haematopoiesis , stat5 , leptin receptor , signalling , microbiology and biotechnology , cytokine , biology , socs2 , adipocyte , hormone , receptor , signal transduction , endocrinology , suppressor , stat3 , gene , adipose tissue , immunology , biochemistry , stem cell , obesity
The adipocyte‐secreted hormone leptin participates in the regulation of hematopoiesis and enhances proliferation of hematopoietic cells. We used an adaptation of the MAPPIT mammalian two‐hybrid method to study leptin signalling in a hematopoietic setting. We confirmed the known interactions of suppressor of cytokine signalling 3 (SOCS3) and STAT5 with the Y985 and Y1077 motifs of the leptin receptor, respectively. We also provide evidence for novel interactions at the Y1077 motif, including phospholipase C gamma and several members of the SOCS protein family, further underscoring the important role of the Y1077 motif in leptin signalling.