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VHA‐8, the E subunit of V‐ATPase, is essential for pH homeostasis and larval development in C. elegans
Author(s) -
Ji Yon Ju,
Choi Kyu Yeong,
Song Hyun-Ok,
Park Byung-Jae,
Yu Jae-Ran,
Kagawa Hiroaki,
Song Woo Keun,
Ahnn Joohong
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.067
Subject(s) - homeostasis , protein subunit , atpase , chemistry , microbiology and biotechnology , v atpase , biology , biochemistry , enzyme , gene
Vacuolar H + ‐ATPase (V‐ATPase) is an ATP‐dependent proton pump, which transports protons across the membrane. It is a multi‐protein complex which is composed of at least 13 subunits. The Caenorhabditis elegans vha‐8 encodes the E subunit of V‐ATPase which is expressed in the hypodermis, intestine and H‐shaped excretory cells. VHA‐8 is necessary for proper intestinal function likely through its role in cellular acidification of intestinal cells. The null mutants of vha‐8 show a larval lethal phenotype indicating that vha‐8 is an essential gene for larval development in C. elegans . Interestingly, characteristics of necrotic cell death were observed in the hypodermis and intestine of the arrested larvae suggesting that pH homeostasis via the E subunit of V‐ATPase is required for the cell survival in C. elegans .