Premium
Transcription regulation of the α‐glucanase gene agn1 by cell separation transcription factor Ace2p in fission yeast
Author(s) -
Dekker Nick,
de Haan Annett,
Hochstenbach Frans
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.061
Subject(s) - schizosaccharomyces pombe , transcription factor , biology , schizosaccharomyces , transcription (linguistics) , yeast , gene , promoter , reporter gene , sequence motif , microbiology and biotechnology , genetics , saccharomyces cerevisiae , gene expression , linguistics , philosophy
During the final stage of the cell division cycle in the fission yeast Schizosaccharomyces pombe , transcription factor Ace2p activates expression of genes involved in the separation of newly formed daughter cells, such as agn1 + , which encodes the α‐glucanase Agn1p. The agn1 promoter contains three copies of the nucleotide sequence motif CCAGCC, whose presence seems to correlate with Ace2p‐mediated transcription activation. Here, we describe a simple plate‐based assay utilizing as a reporter the secreted glucoamylase of Arxula adeninivorans to investigate the function of this motif. We show that not all three repeats, but only the two most proximal to the transcription start point, act as an upstream activating sequence (UAS). Finally, we demonstrate that this UAS is essential for agn1 promoter activity in vivo.