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Platelet‐activating factor‐induced NF‐κB activation enhances VEGF expression through a decrease in p53 activity
Author(s) -
Ko Hyun-Mi,
Jung Hae Hyun,
Seo Kook Heon,
Kang Yeong-Rim,
Kim Han-A,
Park Sung Jun,
Lee Hern-Ku,
Im Suhn-Young
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.042
Subject(s) - transfection , vascular endothelial growth factor , nf κb , platelet activating factor , tumor necrosis factor alpha , chemistry , platelet , endocrinology , microbiology and biotechnology , vegf receptors , cancer research , biology , medicine , signal transduction , biochemistry , immunology , gene
We investigated the role of p53 in nuclear factor (NF)‐κB dependent, platelet‐activating factor (PAF)‐induced vascular endothelial growth factor (VEGF) expression. Transfected NF‐κB subunits in ECV304 cells increased the tumor necrosis factor‐α promoter activity, which was completely inhibited by p53. Transfected p53 increased p53RE promoter activity, which was completely inhibited by NF‐κB subunits, indicating that cross‐regulation occurs between NF‐κB and p53. PAF‐induced increase in VEGF expression was correlated with decreased p53 activity. These data suggest that NF‐κB‐dependency of the PAF‐induced increase in VEGF expression is due to decreased p53 activity, which is reciprocally regulated by increased NF‐κB activity.