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Molecular imprinted polymer with cloned bacterial protein template enriches authentic target in cell extract
Author(s) -
Zhao Zhuo,
Wang Chunhong,
Guo Minjie,
Shi Linqi,
Fan Yunge,
Long Yi,
Mi Huaifeng
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.037
Subject(s) - polymer , molecularly imprinted polymer , polymerization , template , chemistry , adsorption , small molecule , target protein , molecule , selective adsorption , combinatorial chemistry , molecular imprinting , biophysics , biochemistry , chromatography , nanotechnology , materials science , biology , organic chemistry , selectivity , gene , catalysis
Here we describe a new method for preparing a protein‐imprinted polymer with a cloned bacterial protein template, which recognizes/adsorbs authentic target protein present at a relatively low level in cell extract. In this work, cloned pig cyclophilin 18 (pCyP18) was used as a template. The template protein was selectively assembled with memory molecules from their library, which consists of numerous limited length polymer chains with randomly distributed recognition sites and immobilizing sites. These assemblies of protein and memory molecules were adsorbed by porous polymeric beads and immobilized by cross‐linking polymerization. After removing the template, binding sites that were complementary to the target protein in size, shape and the position of recognition groups were exposed, and their confirmation was preserved by the cross‐linked structure. The synthesized imprinted polymer was used to adsorb authentic pCyP18 from cell extract, and its proportional content was enriched 300 times.