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Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages
Author(s) -
Nishida Takanori,
Orikasa Yoshitake,
Ito Yukiya,
Yu Reiko,
Yamada Akiko,
Watanabe Kazuo,
Okuyama Hidetoshi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.032
Subject(s) - eicosapentaenoic acid , escherichia coli , oxidative phosphorylation , hydrogen peroxide , chemistry , protein carbonylation , biochemistry , oxidative stress , oxidative damage , fatty acid , polyunsaturated fatty acid , gene
The colony‐forming ability of Escherichia coli genetically engineered to produce eicosapentaenoic acid (EPA) grown in 3 mM hydrogen peroxide (H 2 O 2 ) was similar to that of untreated cells. It was rapidly lost in the absence of EPA. H 2 O 2 ‐induced protein carbonylation was enhanced in cells lacking EPA. The fatty acid composition of the transformants was unaffected by H 2 O 2 treatment, but the amount of fatty acids decreased in cultures of cells lacking EPA and increased in cultures of cells producing EPA, suggesting that cellular EPA is stable in the presence of H 2 O 2 in vivo and may protect cells directly against oxidative damage. We discuss the possible role of EPA in partially blocking the penetration of H 2 O 2 into cells through membranes containing EPA.