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Mycothiol‐dependent mycobacterial response to oxidative stress
Author(s) -
Ung Korine S.E.,
Av-Gay Yossef
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.026
Subject(s) - mycobacterium smegmatis , oxidative stress , mycobacterium bovis , hydrogen peroxide , chemistry , microbiology and biotechnology , oxidative phosphorylation , mycobacterium , redox , biochemistry , biology , bacteria , mycobacterium tuberculosis , medicine , organic chemistry , tuberculosis , genetics , pathology
The effect of exogenous oxidative stress on mycothiol (MSH) levels and redox balance was investigated in mycobacteria. Both the thiol‐specific oxidant diamide and hydrogen peroxide induced up to 75% depletion of MSH to form the disulfide form, mycothione (MSSM), in Mycobacterium bovis BCG. In comparison, Mycobacterium smegmatis , a saprophytic mycobacterium, displays a greater tolerance towards these oxidants, reflected by the lack of fluxes in MSH levels and redox ratios upon oxidative stress treatments. The basal ratio of MSH to MSSM was established to be 50:1 in M. bovis BCG and 200:1 in M. smegmatis .