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Role of thioredoxin‐1 in apoptosis induction by α‐tocopheryl succinate and TNF‐related apoptosis‐inducing ligand in mesothelioma cells
Author(s) -
Freeman Ruth E.,
Neuzil Jiri
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.019
Subject(s) - apoptosis , fas ligand , tumor necrosis factor alpha , thioredoxin , cancer research , ligand (biochemistry) , caspase , chemistry , microbiology and biotechnology , mesothelioma , immunology , biology , programmed cell death , biochemistry , medicine , receptor , oxidative stress , pathology
Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox‐active protein thioredoxin‐1 (Trx‐1) in apoptosis induced in MM cells and their non‐malignant counterparts (Met‐5A) by α‐tocopheryl succinate (α‐TOS) and TNF‐related apoptosis‐inducing ligand (TRAIL). MM cells were susceptible to α‐TOS and less to TRAIL, while Met‐5A cells were susceptible to TRAIL and resistant to α‐TOS. MM cells expressed very low level of the Trx‐1 protein, which was high in Met‐5A cells, while the level of Trx‐1 mRNA was similar in all cell lines. Downregulation of Trx‐1 further sensitised Met‐5A cells to TRAIL but not to α‐TOS. Our data suggest that the role of Trx‐1 in apoptosis modulation is unrelated to its anti‐oxidant properties.