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Chitooligosaccharides inhibit activation and expression of matrix metalloproteinase‐2 in human dermal fibroblasts
Author(s) -
Kim Moon-Moo,
Kim Se-Kwon
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.015
Subject(s) - matrix metalloproteinase , chemistry , zymography , gene expression , fibroblast , dermal fibroblast , chitosan , microbiology and biotechnology , matrix (chemical analysis) , biochemistry , gene , biology , in vitro , chromatography
Recently, much attention has been given to chitosan and its hydrolyzed products due to their diverse biological activities. For the first time here we report the inhibitory effect of chitooligosaccharides (COS) on activation and expression of matrix metalloproteinase‐2 (MMP‐2) in primary human dermal fibroblasts (HDFs). COS with 3–5 kDa exhibited the highest inhibitory effect on MMP‐2 activity in HDFs assessed by gelatin zymography. Interestingly, protein expression of MMP‐2 was also inhibited by COS with same molecular weight. This inhibition was caused by the decrease of the gene expression and transcriptional activity of MMP‐2. Furthermore, it was found that COS repressed the gene expression of c‐fos, a part of AP‐1 transcription factor. These results suggest that COS may play an important role in the prevention and treatment of MMP‐2 mediated several health problems such as metastasis and wrinkle formation.