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A translational enhancer element on the 3′‐proximal end of the Panicum mosaic virus genome
Author(s) -
Batten Jeffrey S.,
Desvoyes Benedicte,
Yamamura Yoshimi,
Scholthof Karen-Beth G.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.04.006
Subject(s) - subgenomic mrna , biology , guide rna , rna , polyadenylation , enhancer , untranslated region , virology , genetics , microbiology and biotechnology , genome , gene , cas9 , gene expression
Panicum mosaic virus (PMV) is a single‐stranded positive‐sense RNA virus in the family Tombusviridae . PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap‐independent mechanism of translation. A 116‐nucleotide translational enhancer (TE) region on the 3′‐untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis ‐active TE to another location, both restored translational competence of the 5′‐proximal sgRNA genes in vitro.