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Involvement of Na + /H + exchanger in the calcium signaling in epimastigotes of Trypanosoma cruzi
Author(s) -
Bollo Mariana,
Bonansea Sergio,
Machado Estela E.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.094
Subject(s) - extracellular , intracellular , carbachol , calcium , signal transduction , chemistry , trypanosoma cruzi , agonist , vacuole , calcium signaling , biochemistry , calcium in biology , protein kinase c , biophysics , microbiology and biotechnology , biology , cytoplasm , receptor , parasite hosting , world wide web , computer science , organic chemistry
We studied the effect of Na + extracellular on Ca 2+ mobilization from intracellular store evoked by carbachol in Trypanosoma cruzi . We report that slow component of Ca 2+ signaling evoked by agonist is dependent on extracellular Na + but not on Ins P 3 increase. Moreover, this Ca 2+ signaling progressively increased when pH of the medium changed from 7.0 to 7.8. In addition, we found that it was regulated by PKC. The agonist was also able to induce the alkalinization of the acidic compartment, and both Ca 2+ signaling and alkalinization were inhibited by the EIPA‐inhibitor of the Na + /H + exchanger. These results demonstrated the alkalinization of acidic vacuoles and PKC are involved in the triggering of the epimastigote Ca 2+ signaling.