Differential involvement of ERK 1‐2  and p38 MAPK  activation on Swiss 3T3 cell proliferation induced by prostaglandin F 2α | Zendy

Dekanty Andrés | Zendy; Giulianelli Sebastián | Zendy; Coso Omar A. | Zendy; Rudland Philip S. | Zendy; Jimenez de Asua Luis | Zendy

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Differential involvement of ERK 1‐2 and p38 MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F 2α

Author(s) -

Dekanty Andrés,

Giulianelli Sebastián,

Coso Omar A.,

Rudland Philip S.,

Jimenez de Asua Luis

Publication year - 2006

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2006.03.075

Subject(s) - cyclin d1 , mapk/erk pathway , kinase , signal transduction , microbiology and biotechnology , 3t3 cells , cell cycle , phosphorylation , retinoblastoma protein , prostaglandin f2alpha , transfection , chemistry , cyclin d , cyclin dependent kinase , biology , prostaglandin , gene , biochemistry

Prostaglandin F 2α (PGF 2α ) induces cyclin D 1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF 2α induces extracellular‐signal‐regulated kinase (ERK 1‐2 ) and p38 MAPK activation, and inhibition of any of these signaling pathways completely blocks PGF 2α ‐stimulated DNA synthesis. We also show that ERK 1‐2 , but not p38 MAPK activation is required to induce cyclin D 1 expression, strongly suggesting that the concerted action of cyclin D 1 gene expression and other events are required to induce complete phosphorylation of retinoblastoma protein and S‐phase entry in response to PGF 2α .

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