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Expression of the Arabidopsis high‐affinity hexose transporter STP13 correlates with programmed cell death
Author(s) -
Nørholm Morten H.H.,
Nour-Eldin Hussam H.,
Brodersen Peter,
Mundy John,
Halkier Barbara A.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.064
Subject(s) - arabidopsis thaliana , biology , programmed cell death , arabidopsis , microbiology and biotechnology , green fluorescent protein , symporter , mutant , biochemistry , transporter , gene , apoptosis
We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana membrane protein, STP13, as a high affinity, hexose‐specific H + ‐symporter. Studies with kinase activators suggest that it is negatively regulated by phosphorylation. STP13 promoter GFP reporter lines show GFP expression only in the vascular tissue in emerging petals under non‐stressed conditions. Quantitative PCR and the p STP13 ‐GFP plants show induction of STP13 in programmed cell death (PCD) obtained by treatments with the fungal toxin fumonisin B1 and the pathogen Pseudomonas syringae . A role for STP13 in PCD is supported by microarray data from e.g. plants undergoing senescence and a strong correlation between STP13 transcripts and the PCD phenotype in different accelerated cell death ( acd11 ) mutants.