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Expression of pannexin family of proteins in the retina
Author(s) -
Dvoriantchikova Galina,
Ivanov Dmitry,
Panchin Yuri,
Shestopalov Valery I.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.026
Subject(s) - pannexin , retina , biology , in situ hybridization , microbiology and biotechnology , retinal , gene isoform , inner plexiform layer , gap junction , messenger rna , neuroscience , anatomy , genetics , gene , biochemistry , intracellular , connexin
Expression of the Panx1 and Panx2 members of the pannexin family of gap junction proteins was studied in the retina by in situ hybridization and qRT‐PCR. Both pannexins showed robust expression across the retina with predominant accumulation in the retinal ganglion cells (RGCs). In concordance, immunohistochemical analysis showed accumulation of the Panx1 protein in RGCs, amacrine, horizontal cells and their processes. Two Panx1 isoforms were detected: a ubiquitously expressed 58 kDa protein, and a 43 kDa isoform that specifically accumulated in the retina and brain. Our results indicated that Panx1 and Panx2 are abundantly expressed in the retina, and may therefore contribute to the electrical and metabolic coupling, or to signaling between retinal neurons via the secondary messengers.