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Inactivation of the extrinsic subunit of photosystem II, PsbU, in Synechococcus PCC 7942 results in elevated resistance to oxidative stress
Author(s) -
Balint Ido,
Bhattacharya Jyotirmoy,
Perelman Alexander,
Schatz Daniella,
Moskovitz Yona,
Keren Nir,
Schwarz Rakefet
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.020
Subject(s) - photosystem ii , mutant , protein subunit , oxidative stress , photosystem i , biophysics , oxidative phosphorylation , reactive oxygen species , photosynthesis , chemistry , synechococcus , electron transfer , wild type , microbiology and biotechnology , biochemistry , biology , photochemistry , cyanobacteria , genetics , gene , bacteria
PsbU is a subunit of the extrinsic complex attached to the core of photosystem II. A PsbU‐mutant of Synechococcus PCC 7942 was isolated based on its elevated resistance to externally applied oxidative stress. PsbU‐mutant exhibits fast rates of degradation of the photosystem II core protein, D1, under sub‐saturating as well as high‐light conditions. While forward electron transfer is not affected, back electron flow is severely impaired in the mutant. We suggest that impairment of psbU results in production of reactive‐oxygen‐species, which trigger antioxidative mechanisms even under standard growth conditions. Accordingly, when challenged with external oxidative stress, these cells are more resistant than wild type cells.