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Soluble cytochrome c ‐554, CycA, is not essential for photosynthetic electron transfer in Chlorobium tepidum
Author(s) -
Tsukatani Yusuke,
Miyamoto Ryo,
Itoh Shigeru,
Oh-oka Hirozo
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.016
Subject(s) - cytochrome , mutant , oxidoreductase , electron transfer , cytochrome c , photosynthetic reaction centre , cytochrome b , chemistry , cytochrome b6f complex , biochemistry , biology , photochemistry , gene , mitochondrion , enzyme , mitochondrial dna
We constructed a mutant lacking soluble cytochrome c ‐554 (CycA) by disruption of the cycA gene in the green sulfur bacterium Chlorobium tepidum . The mutant grew phototrophically with a growth rate slower than that of the wild type, suggesting that CycA is not essential for photosynthetic electron transfer even though CycA is known to work as an electron donor to the reaction center. The re‐reduction of photo‐oxidized cytochrome c z by quinol oxidoreductase was inhibited almost completely by the addition of stigmatellin in the mutant cells. This result indicates that, in the mutant cells, the linear electron transfer can occur from the quinol oxidoreductase to cytochrome c z , and to reaction center P840 with no participation of CycA.