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Suppression of inducible nitric oxide synthase by 10–23 DNAzymes in murine macrophage
Author(s) -
Chaudhury Indrajit,
Raghav Sunil K.,
Gautam Hemant K.,
Das Hasi R.,
Das Rakha H.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.03.004
Subject(s) - deoxyribozyme , nitric oxide synthase , chemistry , nitric oxide , macrophage , microbiology and biotechnology , biochemistry , biology , dna , organic chemistry , in vitro
iNOS mRNA of J774 murine macrophage cells was cleaved by 10–23 DNAzymes. DNAzyme target site I or translation initiation site and site II have computer predicted (MFOLD) secondary structures but site III has no secondary structure. All the three DNAzymes cleaved the short transcripts generated from cloned DNA almost with equal efficiency while cleavage efficiency is higher at site III than the other two sites on isolated iNOS mRNA. Interestingly, at intracellular level, DNAzyme targeted at translation initiation codon (site I) having secondary structure cleaved iNOS mRNA, and suppressed its activity and protein expression more efficiently than that targeted at sites II and III.