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Decreased ANT content in Zucker fatty rats: Relevance for altered hepatic mitochondrial bioenergetics in steatosis
Author(s) -
Teodoro João,
Rolo Anabela P.,
Oliveira Paulo J.,
Palmeira Carlos M.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.078
Subject(s) - bioenergetics , adenine nucleotide translocator , oxidative phosphorylation , mitochondrion , voltage dependent anion channel , ant , medicine , steatosis , endocrinology , phosphorylation , biology , respiration , chemistry , fatty liver , biochemistry , anatomy , disease , bacterial outer membrane , ecology , escherichia coli , gene
Mitochondria from Zucker fatty (ZF) rats (a model for fatty liver disease) showed a delay in the repolarization after a phosphorylative cycle and a decrease on state 3 respiration, suggesting alterations at the phosphorylative system level. The ATPase activity showed no differences between control and ZF rats, implying alterations in other components of the phosphorylative system. A pronounced depletion in the content of the adenine nucleotide translocator (ANT) was observed by Western blotting, while no alterations were found in the mitochondrial voltage‐dependent anion channel content. These data suggest that hepatic accumulation of fat impairs mitochondrial function, reflecting the loss of oxidative phosphorylation capacity caused by a decrease in the ANT content.