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N ‐Myristoyltransferase 2 expression in human colon cancer: Cross‐talk between the calpain and caspase system
Author(s) -
Selvakumar Ponniah,
Smith-Windsor Erin,
Bonham Keith,
Sharma Rajendra K.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.076
Subject(s) - western blot , calpain , signal transduction , enzyme , apoptosis , colorectal cancer , kinase , microbiology and biotechnology , chemistry , biology , biochemistry , cancer , gene , genetics
A number of viral and eukaryotic proteins which undergo a lipophilic modification by the enzyme N ‐myristoyltransferase (NMT: NMT1 and NMT2) are required for signal transduction and regulatory functions. To investigate whether NMT2 contributes to the pathogenesis of colorectal carcinoma, we observed a higher expression of NMT2 in most of the cases of cancerous tissues compared to normal tissues (84.6% of cases; P < 0.05) by Western blot analysis. Furthermore, protein–protein interaction of NMTs revealed that m ‐calpain interacts with NMT1 while caspase‐3 interacts with NMT2. Our findings provide the first evidence of higher expression of NMT2 in human colorectal adenocarcinomas and the interaction of both forms of NMT with various signaling molecules.