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GABP complex regulates transcription of eIF6 (p27 BBP ), an essential trans‐acting factor in ribosome biogenesis
Author(s) -
Donadini Alessandra,
Giacopelli Francesca,
Ravazzolo Roberto,
Gandin Valentina,
Marchisio Pier Carlo,
Biffo Stefano
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.068
Subject(s) - ribosome biogenesis , transcription factor , microbiology and biotechnology , biology , biogenesis , ribosome , genetics , gene , rna
Eukaryotic initiation factor 6 (eIF6, alias p27 BBP ) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA‐binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.