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Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor
Author(s) -
Do Hyun-Jin,
Song Hyuk,
Yang Heung-Mo,
Kim Dong-Ku,
Kim Nam-Hyung,
Kim Jin-Hoi,
Cha Kwang-Yul,
Chung Hyung-Min,
Kim Jae-Hwan
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.049
Subject(s) - nuclear localization sequence , nls , nuclear transport , transcription factor , subfamily , microbiology and biotechnology , nuclear protein , biology , dna binding domain , ets transcription factor family , mutant , cell nucleus , nucleus , computational biology , genetics , gene
We investigated nuclear localization signal (NLS) determinants within the AT‐hook and ETS DNA‐binding domains of murine Elf3 (mElf3), a member of the subfamily of epithelium‐specific ETS transcription factors. Deletion mutants containing the AT‐hook, ETS domain or both localized strictly in the nucleus, suggesting that these individual domains contain independent NLS motif(s). Within the AT‐hook domain, four basic residues ( 244 KRKR 247 ) were critical for strong NLS activity, and two potent bipartite NLS motifs (236–252 and 249–267) were sufficient for nuclear import of mElf3, although less efficient than the full domain. In addition, one stretch of basic residues ( 318 KKK 320 ) within the ETS domain appears to be essential for mElf3 nuclear localization. Taken together, mElf3 contains multiple NLS motifs, which may function cooperatively to effect efficient nuclear transport.