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Normal specification of the extraembryonic lineage after somatic nuclear transfer
Author(s) -
Kishigami Satoshi,
Hikichi Takafusa,
Van Thuan Nguyen,
Ohta Hiroshi,
Wakayama Sayaka,
Bui Hong-Thuy,
Mizutani Eiji,
Wakayama Teruhiko
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.036
Subject(s) - somatic cell , lineage (genetic) , microbiology and biotechnology , biology , chemistry , genetics , gene
To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail‐tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4‐expressing blastocysts were as low as 50% and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (>90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained.