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Silencing of filamin A gene expression inhibits Ca 2+ ‐sensing receptor signaling
Author(s) -
Huang Chunfa,
Wu Zhenzhen,
Hujer Kristine M.,
Miller R. Tyler
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.035
Subject(s) - gene silencing , filamin , microbiology and biotechnology , chemistry , signal transduction , gene , receptor , gene expression , biology , biochemistry , cell , cytoskeleton
Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca 2+ ‐sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.