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Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco
Author(s) -
Ning Jue,
Peng Xiong-Bo,
Qu Liang-Huan,
Xin Hai-Ping,
Yan Ting-Ting,
Sun Mengxiang
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.028
Subject(s) - zygote , biology , suppression subtractive hybridization , transcriptome , maternal to zygotic transition , nicotiana tabacum , human fertilization , embryogenesis , embryo , gene , microbiology and biotechnology , genetics , cell division , gene expression , cdna library , cell
We applied suppression subtractive hybridization and mirror orientation selection to compare gene expression profiles of isolated Nicotiana tabacum cv SR1 zygotes and egg cells. Our results revealed that many differentially expressed genes in zygotes were transcribed de novo after fertilization. Some of these genes are critical to zygote polarity and pattern formation during early embryogenesis. This suggests that the transcriptome is restructed in zygote and that the maternal‐to‐zygotic transition happens before the first zygotic division, which is much earlier in higher plants than in animals. The expressed sequence tags used in this study provide a valuable resource for future research on fertilization and early embryogenesis.