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The alkaline transition of blue copper proteins, Cucumis sativus plastocyanin and Pseudomonas aeruginosa azurin
Author(s) -
Sakurai Takeshi
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.023
Subject(s) - plastocyanin , azurin , cucumis , copper protein , chemistry , electron paramagnetic resonance , copper , photochemistry , electron transfer , biochemistry , nuclear magnetic resonance , organic chemistry , biology , botany , physics , photosystem i , chloroplast , gene
Autoreduction of Cucumis sativus plastocyanin and Pseudomonas aeruginosa azurin took place at alkaline pHs, having been accompanied by the decrease in the intensities of the charge transfer band, Cys‐S − (π) → Cu(II) at 597 and 626 nm, and the Cu(II)‐EPR signals with small A II values of 6.5 × 10 −3 and 5.3 × 10 −3 cm −1 for plastocyanin and azurin, respectively. Further, an extra Cu(II)‐EPR signal with a large A II value of 21 × 10 −3 cm −1 also reversibly emerged with increasing pH. Plastocyanin and azurin are in an equilibrium of the three forms at alkaline pHs.