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Androgen receptor auto‐regulates its expression by a negative feedback loop through upregulation of IFI16 protein
Author(s) -
Alimirah Fatouma,
Chen Jianming,
Xin Hong,
Choubey Divaker
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.015
Subject(s) - lncap , downregulation and upregulation , androgen receptor , cell growth , prostate cancer , microbiology and biotechnology , biology , regulator , receptor , cancer research , chemistry , gene , cancer , genetics
Expression of androgen receptor (AR) in prostate epithelial cells is thought to regulate cell proliferation, differentiation, and survival. However, the molecular mechanisms remain unclear. We report that re‐expression of AR in PC‐3 human prostate cancer cell line resulted in upregulation of IFI16 protein, a negative regulator of cell growth. We found that the IFI16 protein bound to AR in a ligand‐dependent manner and the DNA‐binding domain (DBD) of the AR was sufficient to bind IFI16. Furthermore, re‐expression of IFI16 protein in LNCaP prostate cancer cells, which do not express IFI16 protein, resulted in downregulation of AR expression and an inhibition of the expression of AR target genes. Our observations identify a role for IFI16 protein in AR‐mediated functions.