Premium
Enhanced repair of DNA interstrand crosslinks in S phase
Author(s) -
Mladenova Veronika,
Russev George
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.009
Subject(s) - transfection , dna , hela , plasmid , cell cycle , fluorescence microscope , microbiology and biotechnology , chemistry , dna replication , dna repair , fluorescence , cell , matrix (chemical analysis) , phase (matter) , biophysics , biology , biochemistry , gene , chromatography , physics , organic chemistry , quantum mechanics
Hela cells synchronized in G1 and S phases of the cell cycle were transfected with pEGFP crosslinked with trioxsalen. Twelve hours later the number of fluorescent cells was determined by fluorescent microscopy. Cells in S phase have repaired 0.2–0.3 ICL/kb over the 12 h period, while cells in G1 phase repaired interstrand crosslinks much more poorly. The crosslinked plasmids were efficiently recruited to the nuclear matrix both in G1 phase and S‐phase, which showed that the poor repair of G1 cells was a result of a lack of DNA replication rather than of a lack of matrix attachment.