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A GABA A receptor of defined subunit composition and positioning: Concatenation of five subunits
Author(s) -
Baur Roland,
Minier Frédéric,
Sigel Erwin
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.02.002
Subject(s) - receptor , protein subunit , interleukin 5 receptor alpha subunit , xenopus , cys loop receptors , biochemistry , chemistry , enzyme linked receptor , concatenation (mathematics) , g alpha subunit , microbiology and biotechnology , biology , combinatorics , acetylcholine receptor , mathematics , gene , nicotinic acetylcholine receptor
We show that the five subunits of a γ‐aminobutyric acid type A receptor (GABA A receptor) can be concatenated to yield a functional receptor. This concatenated receptor α 1 –β 2 –α 1 –γ 2 –β 2 has the advantage of a known subunit arrangement. Most of its functional properties are not significantly different from a receptor formed by individual subunits. Extent of expression amounted to about 40% of that of non‐concatenated receptors in Xenopus oocytes, after injection of oocytes with comparable amounts of cRNA coding for concatenated and non‐concatenated receptors. The ability to express receptors consisting of five subunits enables detailed studies of GABA A receptor subtype selective compounds.