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Modulating the splicing activity of Tetrahymena ribozyme via RNA self‐assembly
Author(s) -
Hasegawa Sumitaka,
Rao Jianghong
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.01.090
Subject(s) - ribozyme , tetrahymena , vs ribozyme , mammalian cpeb3 ribozyme , rna splicing , hairpin ribozyme , chemistry , microbiology and biotechnology , rna , trans splicing , biology , biochemistry , gene
The internal guiding sequence (IGS) is normally located at the 5′ end of trans ‐splicing ribozymes that are derived from the Tetrahymena group I intron, and is required for the recognition of substrate RNAs and for trans ‐splicing reactions. Here, we separated the Tetrahymena group I intron at the L2 loop to produce two fragments: the IGS‐containing substrate, and the IGS‐lacking ribozyme. We show here that two fragments can complex not through the IGS interaction but under the guidance of appended interacting nucleotides, and perform trans ‐splicing. The splicing reactions took place both in vitro and in mammalian cells, and the spliced mRNA product from the self‐assembled ribozyme complex can be translated into functional proteins in vivo. The splicing efficiency was dependent on the length of appending nucleotides.