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Conserved cis ‐elements in the Xenopus red opsin promoter necessary for cone‐specific expression
Author(s) -
Babu S.,
McIlvain V.,
Whitaker S.L.,
Knox B.E.
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.01.080
Subject(s) - opsin , xenopus , biology , transgene , conserved sequence , gene , tetrapod (structure) , genetics , rhodopsin , microbiology and biotechnology , retinal , base sequence , biochemistry , paleontology
The long‐wavelength sensitive (red) opsin genes encode proteins which play a central role in daytime and color vision in vertebrates. We used transgenic Xenopus to identify 5′ cis ‐elements in the red cone opsin promoter necessary for cone‐specific expression. We found a highly conserved extended region (−725 to −173) that was required for restricting GFP transgene expression to cones. We further identified a short element (5′‐CCAATTAAGAGAT‐3′) highly conserved amongst tetrapods, including humans, necessary to restrict expression to cones in the retina. These results identify novel conserved elements that regulate spatial expression of tetrapod red cone opsin genes.