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The effect of high‐fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3‐ablated mice
Author(s) -
Hoeks Joris,
Hesselink Matthijs K.C.,
Sluiter Wim,
Schaart Gert,
Willems Jodil,
Morrisson Alastair,
Clapham John C.,
Saris Wim H.M.,
Schrauwen Patrick
Publication year - 2006
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2006.01.059
Subject(s) - ucp3 , intramuscular fat , lipid peroxidation , chemistry , medicine , endocrinology , uncoupling protein , cd36 , fatty acid , lipid metabolism , biochemistry , biology , enzyme , adipose tissue , gene , brown adipose tissue
UCP3 −/− mice showed ∼3‐fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild‐type littermates. Remarkably, this difference was no longer apparent on the high‐fat diet. However, upon high‐fat feeding, intramuscular triacylglycerol levels were ∼50% lower in UCP3 −/− mice, in comparison to UCP3 +/+ animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3 −/− and UCP3 +/+ mice.